Techniques in stem cell research

Characterization of stem cell cultures has two main purposes. Monitoring the integrity of the cell's genome and tracking the expression of proteins associated with changes in pluripotency of epigenetic profiles. Proteome analysis ensures that cells express the factors necessary to maintain pluripotency. Confirmation of differentiation status by analysis of important genetic and protein markers ensures correct cell type identification and proliferation.

  • Stem cell characterization
  • Karyotyping
  • Fluorescence in situ hybridization (FISH)
  • Comparative genomic hybridization

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